The BioCam X from 3Brain is a high resolution system for in-vitro
electrophysiological imaging using a Microelectrode Array (MEA) Biochip. Designed
to allow simultaneous recordings from a total of 4096 electrodes sampled at 18 kHz
per electrode, the BioCam X enables effective monitoring of thousands of
neurons. This system finds application in human stem cell-derived neurons,
acute brain slices, drug discovery, plasticity studies, cardiomyocyte cultures
and optogenetics-combined studies.
At the core of the MEA Biochip is an active monolithic CMOS device packaged
onto a substrate together with a glass reservoir enabling on-chip amplification
and multiplexing, which allows long term, continuous, and simultaneous
recording of electrophysiological signals. The wide opening of the reservoir
allows concurrent visual inspection of the experiment using a microscope as
well as integrating other recording techniques such as patch clamp and voltage
The BioCam X allows high sampling frequency and a user selectable
recording bandwidth for any type of signal from slow field potentials to single
action potentials. The three Biochip 4096 series provide different spatial
resolutions and recording areas. This allows full monitoring of
electrophysiological signals in a field of view up to ~26 mm_ from a large
variety of biological preparations ranging from cell cultures to intact tissue
such as brain slices and explanted retina.
Key features of the BioCam X system include ability to store or compress
entire raw signals captured; temperature control; electrical programmable
current-driven stimulator; easy integration with instrumentation such as microscopes,
perfusion and patch-clamp systems; and ability to be interfaced with a laptop.