Dual selective plane illumination microscopy for cleared tissue

Scitech presents the ct-dSPIM by ASI Imaging, designed to image various
cleared tissue samples from whole mouse brains as well as slices of cleared
tissue. The ct-dSPIM is a flexible and easy-to-use implementation of Selective
Plane Illumination Microscopy (SPIM) that allows for dual views (d) of large
samples such as cleared tissue (ct).

The ct-dSPIM is one of many light sheet microscope configurations
possible using ASI’s modular components. ASI manufactures the opto-mechanical
elements, including the motorised stages and 2D galvos for creating and moving
the light sheet. ASI partnered with Special Optics to develop an objective
optimised for light sheet imaging of cleared tissue.

Lasers and sCMOS cameras are required to complete the system, and may also
be purchased from Scitech.

Two objectives are placed at right angles above a sample mounted
horizontally in an open dish, each objective 45¡ from the vertical. A light
sheet is created from one objective and imaged using the other objective. A
stack of images is collected by moving the light sheet through the sample; in
the case of ct-dSPIM, the sample is normally moved through a stationary light
sheet using the XY stage.

For some applications, the 3D information from a single view or stack is
sufficient. For dual-view systems, the role of the two objectives is reversed
to collect another stack from a perpendicular direction. The two datasets can
be computationally merged to yield a 3D dataset with isotropic resolution; the
usual problem of poor axial resolution is overcome by information from the
other view.

The ct-dSPIM offers image acquisition >10^8 voxels/sec; sub-micron
resolution in XYZ (sample-permitting); sample mounting in open dish; image
>5 mm deep into flat samples or up to 12mm radius sphere; media RI range
from 1.33 to 1.56, aqueous or organic media; and a modular and flexible setup.

For more information, please visit the Scitech website www.scitech.com.au or call (03) 9480 4999.

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